Based on the imaging principle of frame transfer CCD sensor, some key problems during photoelectric coversion, charge transferring and electron detecting were discussed, such as pixel response non-uniformity, camera shaking, charge transfer efficiency, the frame transfer smear, etc. The corresponding mathematical model was established. The simulation system was designed based on the platform of VC++ 6.0. At last, based on the experimental method, by changing the CCD parameters, such as the transfer efficiency, exposure time, etc, the simulation results were analyzed. Experimental results showed the validity of simulation system.