The purpose is to assess the the feasibility of femtosecond laser application in deep lamellar cut in rabbit corneas,search for the suitable parameter,and to evaluate the laser′s effectiveness through edema corneas. The sample was put on a three-axis translation stage which was under the control of concentrically ringed program,femtosecond laser (800 nm/50 fs) with different pulse energies was applied to focus on the rabbit corneas.The laser focused on the deep lamellar and produced dissection through the corneas along with the movement of the stage. After the lamellar interface cut,the corneal cut surface was examined by light microscopy and scanning electron microscopy(SEM).The corneas operated by using surgical instruments were used as controls. With the pulse energy of 4.4 μJ and the translation stage speed of 200 μm/s,the laser produced an effective and smooth dissection. While with the energy of 6.4 μJ at the speed of 300 μm/s,the laser made an effective and smooth dissection. Compared with the controls ,the experimental groups were better. The laser could also successfully created a smooth cut at the deepness of 400 μm on the edema cornea at the speed of 200 μm/s and the energy of 6.4 μJ.