• Journal of Atmospheric and Environmental Optics
  • Vol. 19, Issue 1, 38 (2024)
CHEN Zhihao1、2、3, ZHAO Nanjing2、3、*, YIN Gaofang2、3, MA Mingjun1、2、3, DONG Ming1、2、3, HUA Hui1、2、3, and DING Zhichao1、2、3
Author Affiliations
  • 1School of Environmental Science and Optoelectronic Technology, University of Science and Technology of China,Hefei 230026, China
  • 2Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics, HFIPS,Chinese Academy of Sciences, Hefei 230031, China
  • 3Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province , Hefei 230031, China
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    DOI: 10.3969/j.issn.1673-6141.2024.01.003 Cite this Article
    Zhihao CHEN, Nanjing ZHAO, Gaofang YIN, Mingjun MA, Ming DONG, Hui HUA, Zhichao DING. A subsection control method of microfluidic automatic sampling in algal cell microscopic imaging[J]. Journal of Atmospheric and Environmental Optics, 2024, 19(1): 38 Copy Citation Text show less
    Schematic diagram of experiment system
    Fig. 1. Schematic diagram of experiment system
    Schematic diagram of cell detection device. (a) Binarized fluorescence signal; (b) fluorescence signal of algal cell;(c) detection process of algal cell fluorescence
    Fig. 2. Schematic diagram of cell detection device. (a) Binarized fluorescence signal; (b) fluorescence signal of algal cell;(c) detection process of algal cell fluorescence
    Schematic diagram of Injection segment control
    Fig. 3. Schematic diagram of Injection segment control
    Fluorescence signal of single cell
    Fig. 4. Fluorescence signal of single cell
    Probability distribution of particle velocity passing through a section in unit time
    Fig. 5. Probability distribution of particle velocity passing through a section in unit time
    Velocity distribution of algae cells under different volumetric flow rates and fitted curve.(a) 10 μL/min; (b) 20 μL/min; (c) 30 μL/min; (d) 40 μL/min
    Fig. 6. Velocity distribution of algae cells under different volumetric flow rates and fitted curve.(a) 10 μL/min; (b) 20 μL/min; (c) 30 μL/min; (d) 40 μL/min
    Automatic acquisition of algae cell images at different flow rates and microscopy results. (a) 0.24 μL/min;(b) 1.50 μL/min; (c) 6.00 μL/min; (d) images obtained through microscope
    Fig. 7. Automatic acquisition of algae cell images at different flow rates and microscopy results. (a) 0.24 μL/min;(b) 1.50 μL/min; (c) 6.00 μL/min; (d) images obtained through microscope
    Flow rate/(μL·min-1)5102030406080
    Theoretical velocity/(mm·s-1)1.352.715.418.1210.8316.2421.65

    Experimental

    Velocity/(mm·s-1)

    1.112.765.197.8110.1812.1427.21
    Deviation/%18.001.803.983.775.9625.2525.68
    Table 1. Comparison of sample average flow rate and theoretical rate results
    ImageFig.7 (a)Fig.7 (b)Fig.7 (c)Fig.7 (d)
    Velocity/(μL·min-1)0.241.506.000.00
    Average gradient value14.423.161.2114.61
    Deviation/%1.3178.3491.750.00
    Table 2. Comparision of image quality
    Group12345678
    Injection time under segmented control/s61.0058.00173.0036.0076.6785.00146.00156.33
    115.6737.00131.6771.3340.0096.67102.33128.00
    51.3324.3323.33108.6755.67114.3334.67166.33
    Average time under segmented control/s87.22
    Theoretical slow injection time/s275.00
    Improvement/%68.28
    Table 3. Comparison of injection times between segmented control and slow injection
    Zhihao CHEN, Nanjing ZHAO, Gaofang YIN, Mingjun MA, Ming DONG, Hui HUA, Zhichao DING. A subsection control method of microfluidic automatic sampling in algal cell microscopic imaging[J]. Journal of Atmospheric and Environmental Optics, 2024, 19(1): 38
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