A high-speed point target acquisition and tracking measurement method based on laser pulse scanning measurement system is proposed. The constant velocity motion model is used to simulate the target trajectory and target distribution probability; considering pulse laser spot distribution parameters, the theory model of pulse laser scanning capture point target is established. A capture method based on time series method is proposed to solve the number of capture of the target model pulsed laser in one frame scanning process. On the basis of this, the main parameters of the laser distributed scanning spot layout are simulated and analyzed. Then, an optimal scanning method is proposed to improve the capture probability, the capture number and the acquisition time for the characteristics of motion target and laser sensor. The feasibility of optimal scanning method is verified through the semi-physical simulation experiment combined with an example. The proposed method provides the basis for the best matching of the pulse frequency, scanning frequency of the laser tracking system and target motion model.