In order to improve the accuracy and maneuverability of self-contained land navigation system, the application of high-accuracy laser Doppler velocimeter (LDV) in the field of land navigation is studied in this paper. The structure and basic principle of the split reuse-type LDV are expounded. Based on the analysis of the advantages and disadvantages of the inertial navigation system (INS) and LDV which are developed by our own department, the integrated technology of marching initial alignment, dynamic calibration and real-time errors compensation are discussed in detail. Expounded in theory that the attitude correction for marching vehicles, dynamic LDV parameter error estimation and high-precision autonomous navigation can be achieved by introducing velocity into the error equation of inertial navigation system. The results of dynamic vehicle tests show that the navigation error of inertial navigation system decreases from 2000 m to 6 m in 1.2 h without parking. It is even proved by theory and experiment that the performance of land navigation system is greatly improved after the application of high-accuracy LDV in land inertial navigation system.