Min XU, Gaofang YIN, Nanjing ZHAO, Xiang HU, Peng HUANG. Simultaneous bright field and fluorescence dual optical path imaging of phytoplankton[J]. Optics and Precision Engineering, 2023, 31(12): 1725
- Optics and Precision Engineering
- Vol. 31, Issue 12, 1725 (2023)
Fig. 1. Phytoplankton bright-field and fluorescence dual-light simultaneous imaging system
Fig. 2. Diagram of dichroic mirror， filter light transmission rate and LED luminous intensity
Fig. 3. Comparison of imaging results of different combinations
Fig. 4. USAF1951 resolution board image
Fig. 5. Bright-field microscopic images of phytoplankton collected by the system.
Fig. 6. Bright-field and fluorescence images of pediastrum at different exposure times.
Fig. 7. Light field and fluorescence microscopic images of different algal species
Fig. 8. Mean grayscale brightness of fluorescence images of different algal species at 465 nm and 620 nm
Fig. 9. Fusion and comparison of light field and fluorescence microscopic images of different algal species
Fig. 10. Algal cells in bright-field and fluorescence fusion images
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Table 1. Experimental algal species information
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