With the metal powders as the filler materials, the laser welding between the dissimilar materials of Ti3Al-based alloy and GH4169 superalloy is carried out. The microstructures at different areas of the joints are analyzed. The micro-hardness in different cross sectional regions of joints and the tensile strength at the room temperature of the joints are tested. The study results indicate that, when the filler material is only the Ti-Ni-Nb powders, the average tensile strength at the room temperature of the joints is 129 MPa. There are no reaction layers generated in both interfaces between the filler material and the two kinds of base materials, and the welds mainly consist of Ti-Ni-Nb phase, Nb-Ti solid solution and precipitated Nb. The micro-hardnesses at the interfaces between welds and base materials are higher than those of the weld center and the base materials. When the filler material is composed of Ti-Nb/Ti-Ni-Nb/Ni-Cu powders, the tensile strength of the joints increases to 180 MPa. The contents of the main elements in the joints vary gradually along the weld line with the composition variation of the filler materials. The Ti3Al/Ti-Nb interface possesses the relatively high micro-hardness, and the hardnesses in the Ti-Nb and Ti-Ni-Nb regions are higher than that in the Ni-Cu region.