Aiming at the difficulty of Raman spectra quantitative analysis on materials with strong fluorescence background, together with baseline correction, a new normalization method was performed for concentration quantitative analysis of two kinds of solutions which exhibit strong fluorescence background, the methanol solutions with different concentrations and the mixed solutions of ethanol and methanol with different ratios of concentration. Meanwhile, the data fluctuation caused by collecting spectra data at different space-time was investigated by using statistical method of randomized blocks analysis of variance to evaluate the data fluctuation among different sample groups, and the function of our method proposed in this paper to eliminate this data fluctuation was discussed. Experimental results demonstrate that our proposed method can not only obtain satisfied accuracy for Raman spectra quantitative analysis on methanol with strong fluorescence background, with the mean relative error being 4.7%, but also effectively eliminate the data fluctuation among different sample groups, and the relative standard deviation of them was only 4.2%, indicating that it is possible to carry out simple, quick and precise quantitative determination of sample content for the materials with strong fluorescence background.Quantitative analysis