The skin tissues,which dealt separately by low-frequency ultrasound,80% glycerol and both of them,were observed by optical coherence tomography (OCT). The 1-D OCT image of epidermis and under 0.012 mm of the skin tissue surface were quantitative analysis by computer. The results of measurements showed that the low-frequency ultrasound and 80% glycerol not only can change the depth of light penetration of the skin tissue in short-time,but have synergistic effect on optical clearing of skin tissue in vitro.