To overcome some drawbacks of splitting-light path (SLP) for plane and concave grating typed spectrometer, including serious aberration, worse spectral fatness and low diffraction efficiency etc, a novel SLP based on volume phase holographic transmission (VPHT) grating is designed. For this grating, its manufacture and theories are investigated, and its diffraction efficiency is numerically simulated. In order to validate this designed SLP, the spectral scaling experiment is performed and the spectral resolution is better than 2 nm, the calibration equation between the scaling wavelength and corresponding pixels is gotten via linear least square fitting algorithm. It is proved that the wavelength absolute value is about 0.37 nm on the wavelength of 635 nm with the method of averaging multiple measurements, the root-mean-square error (RMSE) of full scaling wavelengths is 0.3 nm. In addition, for wavelengths of 200~800 nm, the relationship between wavelength biases and corresponding diffraction wavelengths is established via polynomial fitting algorithm, which can greatly solve the problem of wavelength calibration in SLP.