To achieve rapid, low-cost, and high-throughput detection of bacteria, an array flexible paper-based Surface-Enhanced Raman Scattering(SERS) detection chip was designed and fabricated. First, silver sol was prepared, and then the isolation area and the array detection area were constructed on paper base using the hydrophobicity of laser printing and carbon powder. Given the hydrophobic property of carbon powder, silver sol droplets were confined to the detection area. At a certain temperature, the silver sol dries naturally to form an array of active SERS detection chip. The paper-based SERS chip was characterized by Rhodamine 6G as probe molecule. The test results show that the detection limit of the chip is 10-8 mol/L, and the RSD of the repeatability test is about 11.85%. The SERS test of Escherichia coli(E. coli) shows that the chip can quickly obtain the Raman characteristic peaks of E. coli without labeling or complicated pretreatment. The advantages of the flexible paper-based SERS chip include simple structure, quick fabrication, and low cost. The array structure can realize simultaneous measurement of multiple parameters, and is expected to be used for direct and rapid detection of pathogenic bacteria.