Sensible and latent heat fluxes exchange between surface and atmosphere is a hot topic in eco-environmental research recent years. A software using large aperture scintillometer (LAS) to measure sensible heat fluxes and transverse wind speed is introduced. To meet demands of the data acquisition and disposal of LAS for measuring sensible fluxes and wind speed, the software for the real-time data collection is designed using PCI8340, a data acquisition card, disposing and displaying signals of LAS on Windows platform. It is convenient for persons to get the experimental results quickly and exactly. In addition, it can run automatically because of the data validity check module of the software.