The contents of six trace elements Ca, Mg, Fe, Mn, Cu and Zn in the medicinal materials of cultivated and wild Rhizoma et Radix Notopterygii vegetated in different months were determined by flame atomic absorption spectrometry (FAAS) with air-acetylene flame, and the feature of trace elements contents and their seasonal dynamics were analyzed using SPSS16.0 analysis software.The recovery rate obtained by standard addition method ranged between 96.33% and 105.25%, and the relative standard deviation was 0.84%~2.98%.This showed that the method had good precision and definition.The study indicated that there are abundant microelements in medicinal materials of cultivated and wild Rhizoma et Radix Notopterygii.The contents of Ca are both highest in the medicinal materials of cultivated and wild Rhizoma et Radix Notopterygii, and the contents of Cu element are lowest; the contents order is Ca>Mg>Fe>Mn>Zn>Cu; the seasonal dynamics of Ca, Mg, Fe, Mn, Cu and Zn had obvious regularity in the medicinal materials of cultivated and wild Rhizoma et Radix Notopterygii.It is concluded that the amount of six elements in wild Rhizoma et Radix Notopterygii are more than the cultivated; the data can provide reference for artificial cultivation of wild herbs.