The high-magnification microscope objective lens (HMOL) have small field and short focal length. Measurement of this short focal length is difficult in a conventional way. The focal length of high-magnification microscope objective can be measured using multiple reference transfer based on magnification method. This method is that an infinity design of low-magnification microscope objective lens (LMOL) is measured, the focal length of the LMOL is obtained, and then using the LMOL instead of collimator to measure the focal length of HMOL. This method is used to measure a Nikon metallographic objective lens (nominal focal length is 4 mm) and the errors are analyzed, testing accuracy is 0.0266 mm, and relative standard deviation is 0.664%. This fact that the choice of the objective lens used for reference transfer will affect the accuracy has been noticed, and a standard for choosing the appropriate benchmark objective is proposed.