To explore the influence of micro-vibration of flywheel components on the imaging quality of a high resolution optical satellite, a flywheel component disturbance model and a whole structure dynamic model of the satellite were established. The ground disturbance of flywheel components was tested, the analysis of the measured data shows that there is series of first harmonics at the first order frequency 50 Hz, and a series of peaks independent on rotation speeds around the 190 Hz and 280 Hz. Then, the unit sine excitation was performed on the satellite, the angular displacement response of the optical axis was obtained. The integration of the angular displacement response and the flywheel measured disturbance data was analyzed. The results show that there are a lot of angular displacement harmonic response frequencies in 50 Hz-80 Hz and 230 Hz-280 Hz, the maximum angular displacement resonance amplitudes are 2.718″ and 2.739″ along the optical axis direction and the vertical direction, and 0.5″ magnitude harmonic amplitude is around 245 Hz. It concludes that the flywheel micro vibration has a great influence on the imaging quality of high resolution optical satellites and the results provide important references for system optimum design and vibration isolation.