• Acta Photonica Sinica
  • Vol. 42, Issue 5, 559 (2013)
MAO Xiaofang* and HAN Rong
Author Affiliations
  • [in Chinese]
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    DOI: 10.3788/gzxb20134205.0559 Cite this Article
    MAO Xiaofang, HAN Rong. Effects of HeNe Laser and Enhanced UltravioletB Radiation on TaRAN1 Protein in Wheat[J]. Acta Photonica Sinica, 2013, 42(5): 559 Copy Citation Text show less

    Abstract

    When the seedlings of wheat‘ML7113 ’were exposed to both HeNe 1aser irradiation(5 mW·mm-2) and enhanced UVB radiated rdiation(10.08 kJ·m-2·d-1). 6 days after the extraction of the wheat leaves the total protein and TaRAN1 of protein in each treatment group,identification of target proteins by SDSPAGE of their conduct the preliminary test and Western Blot and Coomassie brilliant blue method to measure the protein content of four different treatment groups TaRAN1 to do further analysis. The results showed that, enhanced UVB radiation makes wheat TaRAN1 protein electrophoresis band color deepened and contents increased significantly; Separate HeNe laser processing, protein electrophoresis with narrow lighter in color and measured the protein content is significantly reduced, showing inhibition; With HeNe laser irradiation and UVB after radiation treatment, The protein content was significantly lower than the enhanced UVB radiation treatment group, while no obvious difference with the control group. Explain the enhanced UVB radiation, the wheat TaRAN1 protein may be involved in the antistress responses of plants.
    MAO Xiaofang, HAN Rong. Effects of HeNe Laser and Enhanced UltravioletB Radiation on TaRAN1 Protein in Wheat[J]. Acta Photonica Sinica, 2013, 42(5): 559
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