ZnO nano-crystal film with uniform and dense distribution is prepared by radio frequency (RF) sputtering and hydrothermal method, and its luminescence under field-emission electron excitation is obtained with the help of carbon nanotube arrays cathode. The results show that under the excitation of electron beam, the spectrum of ZnO nano-crystal film has two emission peaks, in which the yellow or green peak is based on defect luminescence while the ultraviolet peak originates from intrinsic excitons of ZnO. With the increase of electron beam density, the luminescence intensity of ultraviolet peak increases rapidly. In addition, different from usual ZnO films, the ultraviolet peak of ZnO nano-crystal film spectrum exists red-shift, which reaches violet band.