The purpose of this paper is to study a rare earth doped luminescent material with good up-conversion luminescence performance, which is of great significance in anti-counterfeiting technology. In order to improve the up-conversion luminescence performance of LiYF₄∶Yb³⁺/Ho³⁺ micron crystals, a series of Gd³⁺-doped LiYF₄∶Yb³⁺/Ho³⁺ micron crystals were successfully prepared by hydrothermal synthesis method. XRD and Scanning electron microscope (SEM) were used to characterize the samples' phase purity and crystal morphology. Fluorescence spectra analyzed the upconversion luminescence properties of LiGd_xY_1-xF₄∶Yb³⁺/Ho³⁺ micron crystals under 980nm laser excitation. Firstly, the crystal structure, size, morphology and upconversion luminescence properties of LiGd_xY_1-xF₄∶Yb³⁺/Ho³⁺ micron crystals were investigated. The results show that the XRD diffraction peaks of LiGd_xY_1-xF₄∶Yb³⁺/Ho³⁺ micron crystal samples are exactly corresponding to the characteristic peaks of the tetragonal LiYF₄ standard card (PDF#17-0874) without other peaks. The SEM characterization results show that the crystal morphology is octahedral. It shows that LiGd_xY_1-xF₄∶Yb³⁺/Ho³⁺ micron crystal with pure tetragonal phase was successfully synthesized. Fluorescence spectrum test results showed that the up-conversion luminescence intensity of the samples increased first and then decreased with the increase of Gd³⁺ ion doping concentration and reached the maximum when Gd³⁺ ion doping concentration was 30 mol%. Secondly, the relationship between the up-conversion luminescence performance and the excitation power of Gd³⁺ doping concentration of 30 mol% was further studied, and the excitation power was 0.5~1.5 W. The red and green upconversion luminescence intensity (R/G) ratio of LiGd_0.3Y_0.49F₄∶Yb³⁺/Ho³⁺ micron crystal only changes about 12% with the increase of excitation power. The upconversion luminescence of LiGd_0.3Y_0.49F₄∶Yb³⁺/Ho³⁺ micron crystal does not change obviously with the increase of excitation power, but still emits stable and bright green light. This phenomenon shows that incorporating Gd³⁺ ions greatly improves the up-conversion luminescence performance of the samples, and this stable and efficient luminescence performance guarantees its good anti-counterfeiting performance. Finally, LiYF₄∶Yb³⁺/Ho³⁺ micron crystal powder with Gd³⁺ ion doping concentration of 30 mol% was mixed with screen metal ink in a certain proportion to produce screen anti-counterfeiting ink, and then the anti-counterfeiting logo pattern of “Xi'an” was printed on the glass base by screen printing technology. After drying treatment, under the excitation of 980 nm laser, it emits bright and stable green visible light, and the anti-counterfeiting logo made has the characteristics of high luminous intensity, easy to identify and not easy to fall off, which can be widely used in the field of anti-counterfeiting.