Femtosecond fiber laser at 780 nm for two-photon autofluorescence imaging

Wan Yang; Danlei Wu; Runlong Wu; Guanyu Liu; Bingying Chen; Lishuang Feng; Zhigang Zhang; Aimin Wang

doi:10.3788/COL201917.071405

Journals >Chinese Optics Letters >Volume 17 >Issue 7 >Page 071405 > Article

Chinese Optics Letters
Vol. 17, Issue 7, 071405 (2019)

Femtosecond fiber laser at 780 nm for two-photon autofluorescence imaging

Wan Yang¹, Danlei Wu^1、2, Runlong Wu¹, Guanyu Liu¹, Bingying Chen¹, Lishuang Feng², Zhigang Zhang¹, and Aimin Wang^1、*

Author Affiliations

¹State Key Laboratory of Advanced Optical Communication System and Networks, School of Electronics Engineering and Computer Science, Peking University, Beijing 100871, China

²Key Laboratory of Precision Opto-Mechatronics Technology, Ministry of Education, School of Instrumentation Science & Optoelectronics Engineering, Beihang University, Beijing 100191, China

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DOI: 10.3788/COL201917.071405 Cite this Article Set citation alerts

Wan Yang, Danlei Wu, Runlong Wu, Guanyu Liu, Bingying Chen, Lishuang Feng, Zhigang Zhang, Aimin Wang. Femtosecond fiber laser at 780 nm for two-photon autofluorescence imaging[J]. Chinese Optics Letters, 2019, 17(7): 071405 Copy Citation Text

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Schematic diagram of the laser system. PBS, polarization beam splitter; SM LD, single-mode laser diode; MM LD, multi-mode laser diode; WDM, wavelength division multiplexer; ISO, isolator.

Fig. 1. Schematic diagram of the laser system. PBS, polarization beam splitter; SM LD, single-mode laser diode; MM LD, multi-mode laser diode; WDM, wavelength division multiplexer; ISO, isolator.

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Results of the Er:fiber laser. (a) Calculated nonlinear phase shift and residual TOD in the FCPA system, (b) spectra and intensity autocorrelation traces under different DCF lengths.

Fig. 2. Results of the Er:fiber laser. (a) Calculated nonlinear phase shift and residual TOD in the FCPA system, (b) spectra and intensity autocorrelation traces under different DCF lengths.

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Fig. 3. Experimental results of the frequency doubling. (a) RF spectrum of the oscillator, (b) output average power, (c) optical spectrum, (d) intensity autocorrelation trace of the frequency doubled pulse.

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Fig. 4. Optical spectra of frequency doubling under different DCF lengths.

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Fig. 5. (a) Experimental result of TPAM imaging of the rabbit intestine tissue ex vivo. Image size: 248 μm. Scale bar: 20 μm. (b) Experimental result of TPAM imaging of the human skin in vivo at different depths. Image size: 130 μm. Scale bar: 20 μm.

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Component	Other Fibers	Fiber Stretcher	Grating Compressor
Type	SMF-28	DCF	966 l/mm	600 l/mm
$β_{2}$ ( ${fs}^{2} / mm$ )	$- 22$	170	$- 14871$	$- 3107$
$β_{3}$ ( ${fs}^{3} / mm$ )	127	$- 983$	137369	12085
$β_{3} / β_{2}$ (fs)	$- 5.77$	$- 5.78$	$- 9.24$	$- 3.89$