The seedlings of wheat (Jinmai 8) were exposed to He-Ne laser with 5 mW·mm-2 power density, enhanced ultraviolet-B radiation (UV-B) (10.08 kJ·m-2·d-1) and the combination of He-Ne laser and enhanced UV-B radiation respectively for 5 days. Changes about production rate of superoxide anion, malondialdehyde (MDA) content, ultraviolet absorption value and conductance of exosmic fluid of wheat seedlings, content of soluble protein and chlorophyll were measured to analyze the repair role of He-Ne laser irradiation. The results showed that He-Ne laser irradiation on the wheat seedlings led to the decrease of the production rate of superoxide anion, MDA content and ultraviolet absorption value and conductance of exosmic fluid of wheat seedlings. The content of soluble protein and chlorophyll increased to 76.66% and 1.79 mg/g respectively. It suggested that those changes about production rate of superoxide anion, MDA, ultraviolet absorbing value and conductance, soluble protein and chlorophyll were related to the repair capacity of the wheat seedings. Therefore, the damage of wheat seedlings induced by enhanced UV-B radiation can be repaired partly by He-Ne laser.