A new dondr-π-acceptor-π-donor Schiff base-ferrocene derivative is synthesized and characterized by 1H NMR and MS. The UV-vis absorption spectra of the materials are determined and the third-order optical nonlinearity is measured by optical path of degenerate four-wave mixing (DFWM) at wavelength of 800 nm and response time of 80 fs. The third-order nonlinear optical susceptibilities χ(3) of this compound is 3.67×10-13 esu and the second order hyperpolarizabilities γ of this molecule is 3.49×10-31 esu.The influence of molecular structure on the third-order optical nonlinearity is studied and the long conjugate chain, formation of accepor-donor structure can improve the third-order optical nonlinearity.