Digital signal processing is a course with strong combination of theory and practice. Combined with the characteristics of the remote sensing science and technology and its practical application, a comprehensive course experiment of digital signal processing based on MATLAB is designed. This experiment takes the gravity recovery and climate experiment (GRACE) satellite Level 2 data as the research object. Through the operation of signal spectrum analysis, filter design and processing, it can denoise the stripes efficiently and effectively. This comprehensive experiment covers the main key knowledge points of the digital signal processing course, which is helpful for students to form a relatively complete knowledge system of digital signal processing, and cultivates students’ ability to apply abstract theoretical knowledge to actual remote sensing processing.