By virtue of FE-SEM and EDXA marked by Br element, the relationship between the lignin distribution and growth stress was micro-analyzed by normal wood of plantation Eucalyptus urophylla×E. grandis with different growth stress levels. The results indicated that the lignin contents of different cell wall layers were not related to the growth stress level, and the lignin content (from high level to low level) in sequence was cell corner, compound middle lamellae, S1, S2 and S3; The lignin contents in micro-morphological regions of wood fiber cell wall were decreased with the increase in growth stress and lignification process was delayed.