The growth and spectral properties of Er3+/Yb3+∶Sr3Y2(BO3)4 crystal were reported in this paper. Er3+/Yb3+∶Sr3Y2(BO3)4 crystal with high quality and dimensions of 25 mm×35 mm has been grown by the Czochralski method. The spectral properties of Er3+/Yb3+∶Sr3Y2(BO3)4 were investigated. Based on the Judd-Ofelt (J-O) theory, the spectral parameters were calculated. The oscillator intensity parameters were Ω2=14.10×10-20 cm2,Ω4=1.69×10-20 cm2,Ω6=1.72×10-20 cm2, and the emission cross section was 8.24×10-21 cm2 at 1534 nm. The fluorescence lifetime and radiative lifetime of the Er3+ (4I13/2→4I15/2 transition) were 0.650 ms and 3.873 ms, respectively. The result shows that the Er3+/Yb3+∶Sr3Y2(BO3)4 crystal may be become 1.55 μm laser materials.