A hardware-in-the-loop simulation system for laser jamming in the combat process of imaging guided weapons is established by using the method of accessing the circuit of electro-optical jamming equipments and imaging guidance weapons. The composition, function and principle of hardware-in-the-loop simulation system for laser imaging guided weapon are introduced in detail, the compensation time is determined by matching miss distance, and delay compensation is performed. The difficulties of timing inconsistency and physical delay are solved, and the key technology of hardware-in-the-loop simulation system design is summed up. The system can perform research on hardware-in-the-loop simulation system, and detect the jamming efficiency of laser signals to the imaging weapon system. And the advantages and development of the hardware-in-the-loop simulation system design are pointed out.