The mechanism of the static low magnetic field action on thylakoid solutions was studied by analyzing the Raman spectra and PSII fluorescence of the spinach and Suzhou Green both excited by the light with different wavelength and to the changes in the spectra of the two samples treated by different magnetic fields with the same excitation wavelength. The results indicated that there were a lager number of Hydrogen bonds in the thylakoid solution. The Raman spectra were 531 nm and 532.2 nm and the PSII fluorescence were 689.8 and 686 nm for the spinach and Suzhou Green solution before the treatment by magnetic field. But the Raman spectra changed to 533.2 nm and 532.8 nm, and the PSII fluorescence were 686.8 nm and 685.4 nm for the two sample after the treatment. The static low magnetic field can change the Hydrogen bond energy to a constant value in the two solutions.