Fluorochrome-labeled monoclonal antibody with characteristic M-shaped spectral peak for optical imaging: Dual-labeling versus mixture of fluorochromes

Jan Menke; Thomas Kruewel; Christian Dullin

doi:10.3788/COL201513.051701

Journals >Chinese Optics Letters >Volume 13 >Issue 5 >Page 051701 > Article

Chinese Optics Letters
Vol. 13, Issue 5, 051701 (2015)

Fluorochrome-labeled monoclonal antibody with characteristic M-shaped spectral peak for optical imaging: Dual-labeling versus mixture of fluorochromes

Jan Menke^*, Thomas Kruewel, and Christian Dullin

Author Affiliations

Institute for Diagnostic and Interventional Radiology, University Medical Center, Georg August University, 37075 Goettingen, Germany

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DOI: 10.3788/COL201513.051701 Cite this Article Set citation alerts

Jan Menke, Thomas Kruewel, Christian Dullin. Fluorochrome-labeled monoclonal antibody with characteristic M-shaped spectral peak for optical imaging: Dual-labeling versus mixture of fluorochromes[J]. Chinese Optics Letters, 2015, 13(5): 051701 Copy Citation Text

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Fig. 1. Multispectral fluorescence emission measurements.

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Fig. 2. Fluorescence intensity of Probe C594/680 versus Probe D594+680.

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Probe	Absorption Peak(s) (nm)	Emission Peak(s) (nm)	Cetuximab (Erbitux)	Alexa Fluor 594	Alexa Fluor 680
A594	590	617	1 mg	One vial	—
B680	679	702	1 mg	—	One vial
C594/680	$590 + 679$	$617 + 702$	2 mg	One vial	One vial
$D 594 + 680$	$590 + 679$	$617 + 702$	Mix equal volumes of Probes $A + B$