Fig. 1. Structure and sectional view of the nucleic acid extraction syringe and microfluidic chip detection system

Fig. 2. Schematic diagram of nucleic acid extraction process by a syringe structure nucleic acid extraction system

Fig. 3. Design, physical pictures and working principle of the microfluidic chip. (a) Design of the substrate and primer embedding sequence; (b) physical picture of the microfluidic chip and its working principle. C. alb-L, C. gla-L, C. par-L, and C. tro-L represent the types of primers embedded in the reaction chamber. LAMP reaction reagent is transparent in real, and red pigment is added here to indicate the location of the amplification reagent on the chip. PC, positive control; NC, negative control; No., serial number

Fig. 4. Structural diagram and physical picture of the temperature control module. (a) Hierarchical diagram of the temperature control module; (b) structural diagram of the temperature control module; (c) physical picture of the temperature control module

Fig. 5. Structural diagram and physical picture of the fluorescence detection module. (a) Structural diagram; (b) physical picture

Fig. 6. Diagram of microfluidic chip nucleic acid amplification and detection system

Fig. 7. Physical picture of syringe-microfluidic chip based nucleic acid extraction and detection system

Table 1. Detection analysis of the fully integrated nucleic acid analysis system versus kit method

Table 2. Comparison of clinical test results of fully integrated method and culture method for four fungi