Abstract：A testing system for phosphor luminous characteristics based on integral sphere is proposed so as to show luminous characteristics of phosphor at actual operation circumstances and measure its luminous characteristics accurately. Blue LED is used as an excited source in the system. TEC temperature control system is used to control the temperature of the source effectively. And continuous and stable excited light is provided to excite phosphor illumining. Energy loss is prevented effectively and testing accuracy is enhanced for the direction of source controlled by a light tube and light ray collected by integral sphere. Based on experiments, spectrum power distributions of phosphor excited by blue light with difference light strength and variation rules of parameters such as luminous efficiency, quantum efficiency and light conversion efficiency are got. With the increasing of driving current, a small blue shift appears in blue light peak wavelength for quantum confined Stark effect in blue light chip. Three kinds of efficiency almost show linear decline trend at low current and then approach to constant at high current. Experimental results show that luminous characteristics of phosphor on real LED chip at operation state can be evaluated effectively by the system and method.