Single spectral technology can not comprehensively reflect structural features and sources of dissolved organic matter (DOM), therefore, in this study ultraviolet-visible (UV-Vis) and the three-dimensional excitation emission matrix fluorescence (3D-EEM) spectroscopy is applied to investigate the structure and origin of dissolved organic matters (DOM) from top soils on SongHuaba reservoir in Kunming. The results showed that the characters of all samples UV-visible spectra were similar. The ultraviolet absorbance of DOM samples decreased gradually with the wavelength increasing. There was an obvious shoulder absorption peak in the 250～280 nm band. The parameters of A250/A365 (absorbance at 250 to 365 nm) ranged from 2.59 to 5.21, which were illustrated that soil DOMs were composed of fulvic acid or humic acid with higher molecular weight. SUVA254 (the specific UV absorbance at 254 nm) and SUVA260 (the specific UV absorbance at 260 nm) were 1.19～3.00 and 1.15～2.89, which showed significant correlation, indicating that soil DOMs had a higher degree of humification and more hydrophilic components in this reservoir. The 3D-EEM spectrum revealed that all DOM samples had three special peaks, which were UV fulvic-like fluorescence, visible fulvic-like fluorescence and humic-like fluorescence, but protein-like fluorescence was not special. As a whole, the fluorescence index (FI) was closed to 1.4, which suggested that the soil DOMs in SongHuaba revesior were main from the external import. Autochthonous index (BIX) was less than 1, which suggested that the soil DOMs were affected by human activities and had low inherent character and bioavailability. These results serve to provide a theoretical basis for the further study of the DOMs effect on the migration and transformation of organic or heavy metal contaminants.