Electrical/thermal poling, heat treatment and ultrasonic acid treatment were used to investigate the properties of second harmonic generation in the 1.6Аl2O3-6.1Na2O-24.8B2O3-67.5SiO2(wt-%) pseudoquadruplex glass, together with UV-Vis-NIR optical transmission spectra, scanning electron microscopy (SEM), X-ray dlffiaction (XRD) and Maker fringe measurements. The results show that electrical/thermal poling induces the generation of second harmonic (SH) signal in the glass. When thermal field is constant (280 ℃), the Intensity of SH signal increases with increasing electric field and poling time. Heat treatment induces the combination and growth of the mlcrocrystals in the glass, thus at some angles the strong SH signal appears. Ultxasonic acid treatment induces the formation of thin microcrystals at surface and the strong SH signal appears in the broad range of angles. The existed crystallized phase in this glass is α-cristobalite.