In order to improve the ablation precision,efficiency and the automation of Laser Ablation System(LAS) in Mass Spectrograph(MS),the intelligentization of laser ablation system was studied in MS by taking inert gas isotope-MS as an experimental subject.A two-dimensional sample worktable was redesigned,the automation of graphite furnace injection system was also given.A series of control subroutines were designed to calibrate the vision system.After calibration,the laser ablation system is very accurate,the maximal repeatability error of auto-focus is 0.005 mm.The experimental results show that new system has a higher stability and work efficiency.