A measurement method of short carbon nanotubes by multi-angle depolarized dynamic light scattering is developed to reduce the measurement error from the inaccuracy of the scattering angle by linear fitting of the results at different angles. And the reproducibility of the measurement system is improved by this new method as well. The principle of multi-angle dynamic light scattering measurement of short carbon nanotube is studied, and an experimental measurement system is set up. The signal collecting and processing system is developed by LabVIEW based on a photon counting. The Calibration test is carried out by standard short carbon nanotubes suspensions. The measurement results of the diameter and the length of the samples at 90°, 100°, 110°, 120°, 130° and 150° are compared with that at the single angle (i.e. 90°). It shows that the repeatability error of the diameter and the length measured by the multi-angle depolarized dynamic light scattering method are 6.27 % and 6.36 % respectively, and better than those of the single angle method (i.e. 13.22 % and 12.45 %).