Volumetric imaging enables quantitative and global measurements of a three-dimensional (3D) complex system. It allowed quantitation of molecules in the whole volume of a specimen, and has proven to be in-valuable in the studies of cell metabolism, brain func-tion, and developmental biology. Light-sheet fluores-cence microscopy and optical projection tomography have been reported to image 3D volumes with high resolutions and at high speeds. Such methods, however, usually rely on fluorescent labels for chemical targeting, which could perturb the biological functionality in liv-ing systems.