Fig. 1. Typical OCT images of locust embryo. (a) 3D projection image of locust embryo; 2D B-scan cross-sections of embryonic heart at (b) end of diastole and (c) end of systole

Fig. 2. Flow chart of quantitative detection algorithm for cardiac function parameters

Fig. 3. OCT M-Mode images of 9-day locust embryo. (a) Original image before treatment; (b) M-Mode image after grayscale transformation; (c) M-Mode image after grayscale transformation added with seed point; (d) M-Mode image after threshold segmentation; (e) M-Mode image after morphologic processing; (f) amplitude image of locust embryonic heart; (g) extreme points obtained by peak value extraction from Fig. 3(f)

Fig. 4. Heart rate of locust embryo

Fig. 5. Average maximum EDD and minimum ESD versus hatching time of locust embryonic heart

Fig. 6. Calculation diagrams of maximum EDA and minimum ESA of locust embryonic heart. (a)(f) Original images; (b)(g) images after grayscale transformation; (c)(h) images added with seed point; (d)(i) result images after threshold segmentation; (e)(j) images after morphologic processing

Fig. 7. Average maximum EDA and minimum ESA versus hatching time of locust embryonic heart

Table 1. Scanning parameter setting of OCT