“Heibi” refers to serpentinite-related black nephrites whose main components are actinolite. Electron probe microanalysis (EPMA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) analysis showed that “Heibi” was jasper (actinolite). There are three main peaks in OH vibration of Raman spectrum and infrared spectrum, which to MgMgMg-OH, MgMgFe²⁺-OH(Fe_M1²⁺Mg_M1Mg_M3-OH, Mg_M1Mg_M1Fe_M3²⁺-OH), MgFe²⁺Fe²⁺-OH(Mg_M1Fe_M1²⁺-OH, Fe_M1²⁺Fe_M1²⁺Mg_M3-OH), but differing from the common Hetian jade is that the three main peaks of “Heibi” split in the vibration region of OH (3 600~3 700 cm^-1) in Raman spectrum and frequency doubling vibration region of OH (7 200~7 100 cm^-1) in infrared spectrum. The “Heibi” was divided into five regions: HB-1, HB-2, HB-3, HB-4, HB-5. The in-situ EPMA and Raman spectroscopy analysis that there are main peaks (A, B, C) in the Raman spectrum from 3 600 to 3 700 cm^-1. The main peaks were divided into six secondary peaks (A' and A″, B' and B″, C' and C″), and the average wavenumber difference between the secondary peaks was 5 cm^-1. Previous views on the splitting of the main peak at OH vibration of amphibole are various. Based on the in-situ EPMA data of “Heibi” and previous studies, it is found that the cation distribution at B(M4) position in amphibole is the main reason for the splitting. The B(M4) position of amphibole is not directly connected with the OH at W position, the cations on B (M4) position indirectly affect OH at W position by affecting oxygen bridging atoms of TO₄, thus causing certain changes in OH vibration spectrum. Comparing the crystal-chemical formula of amphibole samples with “Heibi” which have similar splitting spectrum, it is found there are Ca²⁺ and Mn²⁺ distributing in B (M4) position of all samples.While the cation occupation in other positions is various, which proves that the splitting of OH vibration spectrum of “Heibi” is related to the distribution of Ca²⁺ and Mn²⁺ at B(M4). Therefore, it is considered that splitting of OH vibration spectrums in “Heibi” ascribe to occupations of Ca²⁺ and Mn²⁺ in B(M4), the high-wavenumber peaks ascribe to Ca²⁺, and the low-wavenumber peaks ascribe to Mn²⁺, which is A', B', C' ascribe to Ca²⁺, and A″, B″, C″ ascribe to Mn²⁺.