As an ideal saturable absorber material, graphene has attracted widespread attentions, which can be used as saturable absorber in passive mode-locking lasers because of its perfect electronic and optical characteristics. Single-layer graphene film fabricated on the copper foil by using chemical vapor deposition method. The corresponding Raman spectroscopy is measured. The graphene film is transferred to the facet of zirconium oxide of a fiber jumper. The mode-lock Er3+-doped fiber laser with graphene film as saturable absorber is experimentally studied, which has a ring structure and use a fiber Bragg grating with transmissivity of 92% to select output frequency. The laser has a peak wavelength of 1556.7 nm, 3 dB bandwidth of 0.068 nm, pulse repetition rate of 6.042 MHz and threshold of 65 mW.