Journals >Journal of Innovative Optical Health Sciences
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2024
Volume: 17 Issue 1
10 Article(s)
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Research Articles
Laser spectroscopy imaging technique coupled with nanomaterials for cancer diagnosis: A review
Zhengying Peng, Pengkun Yin, Dan Li, Youyuan Chen, Kaiqiang Shu, Qingwen Fan, Yixiang Duan, and Qingyu Lin
Laser spectroscopic imaging techniques have received tremendous attention in the field of cancer diagnosis due to their high sensitivity, high temporal resolution, and short acquisition time. However, the limited tissue penetration of the laser is still a challenge for the in vivo diagnosis of deep-seated lesions. NanoLaser spectroscopic imaging techniques have received tremendous attention in the field of cancer diagnosis due to their high sensitivity, high temporal resolution, and short acquisition time. However, the limited tissue penetration of the laser is still a challenge for the in vivo diagnosis of deep-seated lesions. Nanomaterials have been universally integrated with spectroscopic imaging techniques for deeper cancer diagnosis in vivo. The components, morphology, and sizes of nanomaterials are delicately designed, which could realize cancer diagnosis in vivo or in situ. Considering the enhanced signal emitting from the nanomaterials, we emphasized their combination with spectroscopic imaging techniques for cancer diagnosis, like the surface-enhanced Raman scattering (SERS), photoacoustic, fluorescence, and laser-induced breakdown spectroscopy (LIBS). Applications of the above spectroscopic techniques offer new prospects for cancer diagnosis..
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2330008 (2024)
NIR-II fluorescence imaging in liver tumor surgery: A narrative review
Zihao Liu, Lifeng Yan, Qingsong Hu, and Dalong Yin
In liver tumor surgery, the recognition of tumor margin and radical resection of microcancer focis have always been the crucial points to reduce postoperative recurrence of tumor. However, naked-eye inspection and palpation have limited effectiveness in identifying tumor boundaries, and traditional imaging techniques cIn liver tumor surgery, the recognition of tumor margin and radical resection of microcancer focis have always been the crucial points to reduce postoperative recurrence of tumor. However, naked-eye inspection and palpation have limited effectiveness in identifying tumor boundaries, and traditional imaging techniques cannot consistently locate tumors in real time. As an intraoperative real-time navigation imaging method, NIR fluorescence imaging has been extensively studied for its simplicity, reliable safety, and superior sensitivity, and is expected to improve the accuracy of liver tumor surgery. In recent years, the research focus of NIR fluorescence has gradually shifted from the first near-infrared window (NIR-I, 700–900 nm) to the second near-infrared window (NIR-II, 1000–1700
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2330010 (2024)
Single-cell manipulation by two-dimensional micropatterning
Xuehe Ma, Haimei Zhang, Shiyu Deng, Qiushuo Sun, Qingsong Hu, Yuhang Pan, Fen Hu, Imshik Lee, Fulin Xing, and Leiting Pan
Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape, cytoskeleton and organelle, as well as the nucleus morphology and genetic expression. The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organizCells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape, cytoskeleton and organelle, as well as the nucleus morphology and genetic expression. The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis, cellular standardization, and in vivo environment mimicking. Here, we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques, including photolithographic micropatterning and soft lithography micropatterning. Moreover, we summarize the application of micropatterning technique in controlling cytoskeleton, cell migration, nucleus and gene expression, as well as intercellular communication..
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2330011 (2024)
In vivo label-free measurement of blood flow velocity symmetry based on dual line scanning third-harmonic generation microscopy excited at the 1700 nm window
Hui Cheng, Jincheng Zhong, Ping Qiu, and Ke Wang
Measurement of blood flow velocity is key to understanding physiology and pathology in vivo. While most measurements are performed at the middle of the blood vessel, little research has been done on characterizing the instantaneous blood flow velocity distribution. This is mainly due to the lack of measurement technoloMeasurement of blood flow velocity is key to understanding physiology and pathology in vivo. While most measurements are performed at the middle of the blood vessel, little research has been done on characterizing the instantaneous blood flow velocity distribution. This is mainly due to the lack of measurement technology with high spatial and temporal resolution. Here, we tackle this problem with our recently developed dual-wavelength line-scan third-harmonic generation (THG) imaging technology. Simultaneous acquisition of dual-wavelength THG line-scanning signals enables measurement of blood flow velocities at two radially symmetric positions in both venules and arterioles in mouse brain in vivo. Our results clearly show that the instantaneous blood flow velocity is not symmetric under general conditions..
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2350011 (2024)
Laser speckle contrast imaging to predict the effect of temporary spinal cord stimulation in postherpetic neuralgia patients: A prospective observational study
Xiang Huang, Dacheng Tang, Pan Chen, Lumiao Chen, Xiaohong Li, and Xiaoping Wang
Temporary spinal cord stimulation (tSCS) can effectively reduce the pain and severity of postherpetic neuralgia (PHN). However, there are no effective and objective methods for predicting the effects of tSCS on PHN. Laser speckle contrast imaging (LSCI) is frequently used in neurology to evaluate the effectiveness of tTemporary spinal cord stimulation (tSCS) can effectively reduce the pain and severity of postherpetic neuralgia (PHN). However, there are no effective and objective methods for predicting the effects of tSCS on PHN. Laser speckle contrast imaging (LSCI) is frequently used in neurology to evaluate the effectiveness of treatment. To assess the accuracy of LSCI in predicting the impact of tSCS on PHN, 14 adult patients receiving tSCS treatments for spinal nerve-innervated (C6-T2) PHN participated in this observational study. Visual analog scale (VAS) assessments and LSCI blood flow images of the fingers were recorded after the tSCS procedure. The results showed that the VAS scores of all patients decreased significantly. Moreover, the blood flow index (BFI) values were significantly higher than they were before the procedure. Increased blood flow and pain alleviation were positively correlated. The findings indicated that spinal nerve PHN (C6-T2) was significantly reduced by tSCS. Pain alleviation by tSCS was positively correlated with increased blood flow in the hand. The effect of tSCS on PHN may thus be predicted using an independent and consistent indicator such as LSCI..
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2350014 (2024)
Intravascular photoacoustic and optical coherence tomography imaging dual-mode system for detecting spontaneous coronary artery dissection: A feasibility study
Yongwei Wang, Yuyang Wan, and Zhongjiang Chen
In this work, we present an intravascular dual-mode endoscopic system capable of both intravascular photoacoustic imaging (IVPAI) and intravascular optical coherence tomography (IVOCT) for recognizing spontaneous coronary artery dissection (SCAD) phantoms. IVPAI provides high-resolution and high-penetration images of iIn this work, we present an intravascular dual-mode endoscopic system capable of both intravascular photoacoustic imaging (IVPAI) and intravascular optical coherence tomography (IVOCT) for recognizing spontaneous coronary artery dissection (SCAD) phantoms. IVPAI provides high-resolution and high-penetration images of intramural hematoma (IMH) at different depths, so it is especially useful for imaging deep blood clots associated with imaging phantoms. IVOCT can readily visualize the double-lumen morphology of blood vessel walls to identify intimal tears. We also demonstrate the capability of this dual-mode endoscopic system using mimicking phantoms and biological samples of blood clots in ex vivo porcine arteries. The results of the experiments indicate that the combined IVPAI and IVOCT technique has the potential to provide a more accurate SCAD assessment method for clinical applications..
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2350016 (2024)
Anti-PD1 antibody and not anti-LAG-3 antibody improves the antitumor effect of photodynamic therapy for treating metastatic breast cancer
Shan Long, Yibing Zhao, Yuanyuan Xu, Bo Wang, Haixia Qiu, Hongyou Zhao, Jing Zeng, Defu Chen, Hui Li, Jiakang Shao, Xiaosong Li, and Ying Gu
Photodynamic therapy (PDT) has limited effects in treating metastatic breast cancer. Immune checkpoints can deplete the function of immune cells; however, the expression of immune checkpoints after PDT is unclear. This study investigates whether the limited efficacy of PDT is due to upregulated immune checkpoints and tPhotodynamic therapy (PDT) has limited effects in treating metastatic breast cancer. Immune checkpoints can deplete the function of immune cells; however, the expression of immune checkpoints after PDT is unclear. This study investigates whether the limited efficacy of PDT is due to upregulated immune checkpoints and tries to combine the PDT and immune checkpoint inhibitor to observe the efficacy. A metastatic breast cancer model was treated by PDT mediated by hematoporphyrin derivatives (HpD-PDT). The anti-tumor effect of HpD-PDT was observed, as well as CD4
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2350020 (2024)
Self-confocal NIR-II fluorescence microscopy for multifunctional in vivo imaging
Jing Zhou, Tianxiang Wu, Runze Chen, Liang Zhu, Hequn Zhang, Yifei Li, Liying Chen, and Jun Qian
Fluorescence imaging in the second near-infrared window (NIR-II, 900–1880nm) with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imaging with high spatial resolution. However, the traditional NIR-II fluorescence confocal microscope withFluorescence imaging in the second near-infrared window (NIR-II, 900–1880
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2350025 (2024)
In vivo measurement of NADH fluorescence lifetime in skeletal muscle via fiber-coupled time-correlated single photon counting
Kathryn M. Priest, Jacob V. Schluns, Nathania Nischal, Colton L. Gattis, Jeffrey C. Wolchok, and Timothy J. Muldoon
Nicotinamide adenine dinucleotide (NADH) is a cofactor that serves to shuttle electrons during metabolic processes such as glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation (OXPHOS). NADH is autofluorescent, and its fluorescence lifetime can be used to infer metabolic dynamics in living cells. FibNicotinamide adenine dinucleotide (NADH) is a cofactor that serves to shuttle electrons during metabolic processes such as glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation (OXPHOS). NADH is autofluorescent, and its fluorescence lifetime can be used to infer metabolic dynamics in living cells. Fiber-coupled time-correlated single photon counting (TCSPC) equipped with an implantable needle probe can be used to measure NADH lifetime in vivo, enabling investigation of changing metabolic demand during muscle contraction or tissue regeneration. This study illustrates a proof of concept for point-based, minimally-invasive NADH fluorescence lifetime measurement in vivo. Volumetric muscle loss (VML) injuries were created in the left tibialis anterior (TA) muscle of male Sprague Dawley rats. NADH lifetime measurements were collected before, during, and after a 30
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2350030 (2024)
Optical redox imaging of ANT1-deficient muscles
He N. Xu, Ryan M. Morrow, Min Feng, Huaqing Zhao, Douglas Wallace, and Lin Z. Li
Adenine nucleotide translocator (ANT) is a mitochondrial protein involved in the exchange of ADP and ATP across the mitochondrial inner membrane. It plays a crucial role in cellular energy metabolism by facilitating the transport of ATP synthesized within the mitochondria to the cytoplasm. The isoform ANT1 predominatelAdenine nucleotide translocator (ANT) is a mitochondrial protein involved in the exchange of ADP and ATP across the mitochondrial inner membrane. It plays a crucial role in cellular energy metabolism by facilitating the transport of ATP synthesized within the mitochondria to the cytoplasm. The isoform ANT1 predominately expresses in cardiac and skeletal muscles. Mutations or dysregulation in ANT1 have been implicated in various mitochondrial disorders and neuromuscular diseases. We aimed to examine whether ANT1 deletion may affect mitochondrial redox state in our established ANT1-deficient mice. Hearts and quadriceps resected from age-matched wild type (WT) and ANT1-deficient mice were snap-frozen in liquid nitrogen. The Chance redox scanner was utilized to perform 3D optical redox imaging. Each sample underwent scanning across 3–5 sections. Global averaging analysis showed no significant differences in the redox indices (NADH, flavin adenine dinucleotide containing-flavoproteins Fp, and the redox ratio Fp/(NADH+Fp) between WT and ANT1-deficient groups. However, quadriceps had higher Fp than hearts in both groups (
Journal of Innovative Optical Health Sciences
- Publication Date: Jan. 01, 1900
- Vol. 17, Issue 1, 2350032 (2024)
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