The 488 nm, 518 nm and 637 nm lasers in visible wavelength band were used to carry out the light reduction experiment on the graphene oxide samples. Meanwhile the transmittance and resistance of reduced graphene oxide were measured in real time to investigate effect of laser with different wavelengths on reduction of graphene oxide. The results show that the transmittance and resistivity of reduced graphene oxide appear different variation under different wavelengths laser irradiation. When 488 nm laser is used, the sample can be reduced though under the condition of low power density, and the reduction process is in accordance with the photochemical reaction. When using 518 nm and 637 nm laser, only when the laser power density is greater than a certain threshold, can the graphene oxide be reduced; longer the laser wavelength, higher the power threshold; and the reduction process conforms to photothermal reaction. The results can pave a way for the improvement of the patterning process of graphene film.