A new testing method for biomass concentration and sensor design is developed to realize online testing of biomass concentration in the microbial bacterium liquid. The sensor structure theory, optical path, theory analysis and experiment are studied in-depth. First, the optical absorption and optical scattering are related to the optical distance and incidence energy. The change in biomass concentration will cause the change in optical absorption and optical scattering, and then lead to the change in the receiving energy. Based on the physical phenomena and theory, the paper relates the receiving energy to biomass concentration testing, and a new method to measure the biomass concentration is set up. In the experiment, a lamp-house with 760 nm wavelength is chosen. Under the 20uC constant temperature, the experiment is performed on the biomass concentration test. The results indicate that the method is good for testing bacterium liquid concentration, with maximum relative error less than 0.2%. The method, which has merits such as precise online testing, high sensitivity and long life, is a practical new style biomass concentration sensor.