The dispersion of Yb-doped fiber is measured by a spectral interferometric technique. The experimental verification is achieved by comparing the measured data with published data of the Nufern 1060xp fiber and the measurement relative error is 1.36%. The parameters of the experimental system, such as minimum required source bandwidth and minimum fiber length, are introduced and analyzed in the measurement. The minimum required source bandwidth predicted through theoretical calculation at the center wavelength of 1070 nm is 19.3 nm, which perfectly agrees with the experimental value.