Ultraviolet polarization imaging technology has unique advantages in trace detection and space exploration. However, the current research mainly focuses on the ultraviolet polarization imaging technology and the system, and the research on the calibration method of ultraviolet polarization imaging system is relatively lack. In order to solve the calibration problem of uv-polarization imaging system, a systematic calibration experimental method is designed by using the self-developed uv-polarization time-sharing imaging system. By analyzing the design principle of uv-polarization imaging system, the calibration experiment is carried out, and the radiation calibration and polarization calibration of uv-polarization imaging system are realized. The experimental results show that after calibration, the uniformity error of the system is reduced to less than 1% and the measurement error of polarization degree is less than 2%, which meets the requirements of system calibration accuracy. The effectiveness of the calibration method is verified, which is of signicance for the calibration of ultraviolet polarization imaging system.