λ-phage DNA molecules,stained with YOYO-1 fluorescent dye,were put inside sub 100 nm open channels which have width ～40 nm and depth ～60 nm. The DNA molecules were transported along these nanoconduits and the removing information was observed by fluorescence microscopy technique clearly. The movements of DNA molecules in the channels were discussed.Associating with optical molecular imaging,these scale nanostructured fluidic channels would be useful for studying single biomolecules statics as well as the dynamics.