In this paper, a technique based on laser self-mixing effect for acquiring pulse wave profile is proposed and demonstrated. For the characteristics of extremely weak vibration in human pulse waves, a method of multiple reflections with external cavity is designed to improve the measurement accuracy to λ/4. Based on the fringe counting algorithm, one period of pulse wave is successfully reconstructed. The measurement results show that the root mean square error (RMSE) of the reconstructed pulse wave is 0.912 μm at sampling rate of 8 kHz. Besides, the correlation coefficients and heart beats of this method and the traditional photoplethysmography (PPG) measurement are analyzed.