Rewritable collinear holographic image storage was realized in a genetically mutated bacteriorhodopsin BR-D96N film by using its photochromic property. For a BR-D96N film with 3.0 optical density, under 632.8 nm, 700 mW/cm2 recording light (optical reference ratio is about 1∶1.2), the optimum recording time is about 3 s, the optimum reconstruction light intensity is about 50 mW/cm2, and the safetime of the hologram is about 10 min. The experiment shows that, in collinear holographic storage system, simple optical setup, small volume, low environmental effect and the high density storage can be realized; and it is proved that the BR-D96N film has advantages like short storage time, high light sensitivity, high reversibility, long-term stability and easy used, which can be used as a higher sensitive rewritable collinear holographic storage media.