The algorithm for retrieving atmospheric Precipitable Water Vapor(PWV) was introduced by several Moderate Resolution Imaging Spectroradiometer(MODIS) near-IR channels. Three-channel ratio weighting method is applicable for water vapor retrieval over Wuhan by comparing several commonly used algorithms. The credibility of the algorithm is verified in contrast to the EOS water vapor products. The PWV in recent ten years (2001-2011) over Wuhan were analyzed by the three-channel ratio weighting method with the aid of remote sensing image processing software ENVI combined with actual geographical location, climate conditions and urban status of the development. The results show that the PWV over Wuhan has a distinct decreasing trend in the autumn and winter. With the help of scientific statistical software SPSS, unary regression model was used to analyze the relationship between the MODIS derived PWV and ground-based meteorological factors, such as temperature, pressure and relative humidity. The results show that PWV derived from MODIS has positively correlated with temperature, negatively correlated with pressure, has little correlation with relative humidity, except that when the temperature is the same, in which condition the two are positively related.