From June 20 to July 20, 2009, mobile Doppler wind lidar of Ocean University of China was operated in Zhuhai National Climate Observatory (N22°4′54″， E113°12′50″) for atmospheric observation. The lidar system, observation experiment, principle and application of atmospheric boundary layer height detection were introduced. In order to obtain the information of atmospheric boundary layer height, the measured data were processed and analyzed, using the first order derivative method. And the problem of how to detect atmospheric boundary layer height when existing clouds was also discussed. The results show that the height of atmospheric boundary layer is between 0.3 km and 1.2km, and the average height is 0.66 km in Zhuhai from June to July of 2009.