The optical fiber nanoprobe is prepared using spark fused taper and acid corrosion methods. With 3-ami-nopropyltrimethoxysilane coupling, gold nanoparticles are solidified onto the surface of fiber optic and then the optical fiber sensor is prepared using the surface-enhanced Raman spectroscopy (SERS) measurement of the cell solution. The SERS of the esophageal cancer cell solution is measured by direct detection and fiber detection methods. Similar results are obtained by both detection methods. SERS measurement of tissues and organs is done using the optical fiber sensor.