• Advanced Imaging
  • Vol. 1, Issue 1, ()
Guilbert Julien, Negash Awoke, Labouesse Simon, Gigan Sylvain, Sentenac Anne, de Aguiar Hilton Barbosa 
Author Affiliations
  • Laboratoire Kastler Brossel
  • Fresnel Institute Marseille
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    Abstract

    Far-field super-resolution microscopy has unravelled the molecular machinery of biological systems that tolerate fluorescence labelling. Conversely, stimulated Raman scattering (SRS) microscopy provides chemically selective high-speed imaging in a label-free manner by exploiting the intrinsic vibrational properties of specimens. Even though there were various proposals for enabling far-field super-resolution Raman microscopy, the demonstration of a technique compatible with imaging opaque biological specimens has been so far elusive. Here, we demonstrate a single-pixel-based, combined with robust structured illumination, that enables super-resolution in SRS microscopy. The methodology is straightforward to implement and provides label-free super-resolution imaging of thick specimens, therefore paving the way for probing complex biological systems when exogenous labelling is challenging.
    Manuscript Accepted: May. 3, 2024
    Posted: May. 9, 2024