A design method of 32-point real sequence fast Fourier transform (FFT) based on field programmable gate array (FPGA) is presented. Base-2 DIT algorithm and parallel iterative processing scheme are adopted. Cyclone IV series chip of Altera company is chosen and Verilog HDL input method is used to implement design input. It is found that the design can achieve high speed A/D sampling data processing accurately and in real time through contrast of signal tap and Matlab data.